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首页> 外文OA文献 >Molecular characterization of Portuguese populations of the pinewood nematode Bursaphelenchus xylophilus using cytochrome b and cellulase genes
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Molecular characterization of Portuguese populations of the pinewood nematode Bursaphelenchus xylophilus using cytochrome b and cellulase genes

机译：使用细胞色素b和纤维素酶基因对葡萄牙松材线虫Bursaphelenchus xylophilus种群的分子表征

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Bursaphelenchus xylophilus is the causal agent of pine wilt disease and aworldwide pest with high economic impact. Since its first diagnosis in Portugalin 1999, it has been subjected to quarantine measures with impact on foresthealth and ecosystem stability, significantly affecting international trade of woodproducts. The disease was detected in the north and centre of continentalPortugal and, since 2008, the whole country has been considered an affectedarea. Recently, it was detected in Madeira Island. In order to avoid newoutbreaks, it has become of major importance to understand the patterns ofspread, introduction points and to characterize the new populations fromcontinental Portugal and Madeira Island. Mitochondrial cytochrome b (cytb) andparasitic cellulase gene sequences were used to evaluate the geneticrelationships among isolates that could indicate possible origins of the newoutbreaks. Portuguese isolates were compared with isolates from USA, China,Japan and South Korea, in order to investigate possible infection pathways anddisease spread patterns in Portugal. Phylogenetic trees based on both genesshow that Portuguese isolates group with Asian isolates. Isolates from USA arein a separate position in both gene trees. However, the phylogenetic tree basedon the cellulase gene sequences shows higher differentiation among Portugueseisolates than that of cytb. These results agree with those previously obtainedusing inter-simple sequence repeats (ISSR). This was the first study to use cytband cellulase genes to characterize pinewood nematode (PWN) populations.This study suggests that cellulase is a better marker than cytb to study geneticdiversity in B. xylophilus.
机译：松材线虫（Bursaphelenchus xylophilus）是松萎病和全世界害虫的病因，对经济具有很高的影响。自从1999年在葡萄牙首次被诊断以来，它一直受到检疫措施的影响，对森林健康和生态系统的稳定性产生了重大影响，极大地影响了木材产品的国际贸易。在葡萄牙大陆的北部和中部发现了这种疾病，自2008年以来，整个国家被认为是受灾地区。最近，在马德拉岛发现了它。为了避免新的暴发，了解传播的方式，引种点并确定来自葡萄牙大陆和马德拉岛的新种群的特征已变得至关重要。线粒体细胞色素b（cytb）和寄生纤维素酶基因序列被用来评估分离株之间的遗传关系，这可能表明新的爆发可能是起源。将葡萄牙分离株与美国，中国，日本和韩国的分离株进行比较，以研究葡萄牙可能的感染途径和疾病传播方式。基于这两个基因的系统发育树表明，葡萄牙分离株与亚洲分离株成群。来自美国的分离株在两个基因树中都处于不同的位置。然而，基于纤维素酶基因序列的系统发育树显示葡萄牙分离株比cytb更高的分化。这些结果与以前使用简单序列间重复（ISSR）获得的结果一致。这是第一个使用cytband纤维素酶基因来表征松材线虫（PWN）种群的研究。该研究表明，纤维素酶比cytb更好地研究了木糖双歧杆菌的遗传多样性。

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